Activation of Intrarenal Complement System in Mouse Model for Chronic Cyclosporine Nephrotoxicity
Yonsei Medical Journal
;
: 517-525, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-71486
ABSTRACT
PURPOSE:
Local activation of the complement system plays a role in target organ damage. The aim of our study was to investigate the influence of cyclosporine (CsA)- induced renal injury on the complement system in the kidney. MATERIALS ANDMETHODS:
Mice fed a low salt (0.01%) diet were treated with vehicle (VH, olive oil, 1mL/kg/day) or CsA (30mg/kg/day) for one or four weeks. Induction of chronic CsA nephrotoxicity was evaluated with renal function and histomorphology. Activation of the complement system was assessed through analysis of the expression of C3, C4d, and membrane attack complex (MAC), and the regulatory proteins, CD46 and CD55. CsA treatment induced renal dysfunction and typical morphology (tubulointerstitial inflammation and fibrosis) at four weeks.RESULTS:
CsA-induced renal injury was associated with increased the expression of C3, C4d, and MAC (C9 and upregulation of complement regulatory proteins (CD 46 and CD55). Immunohistochemistry revealed that the activated complement components were mainly confined to the injured tubulointerstitium.CONCLUSION:
CsA-induced renal injury is associated with activation of the intrarenal complement system.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fragmentos de Peptídeos
/
Proteínas do Sistema Complemento
/
Complemento C3
/
Imuno-Histoquímica
/
Immunoblotting
/
Complemento C4b
/
Complexo de Ataque à Membrana do Sistema Complemento
/
Ciclosporina
/
Antígenos Comuns de Leucócito
/
Microscopia Confocal
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
2007
Tipo de documento:
Artigo
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