14-bp Insertion/Deletion Polymorphism of the HLA-G Gene in Osteosarcoma Patients
Korean Journal of Pathology
; : 485-490, 2011.
Article
em En
| WPRIM
| ID: wpr-71575
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: The major histocompatibility complex class I, G (human leukocyte antigen-G [HLA-G]) gene plays a vital role in the suppression of immune responses. Recently, a number of studies have reported an association between HLA-G and diseases (pregnancy complications, organ transplantation, and tumors). Some of the studies have revealed that the 14-bp insertion/deletion polymorphism might be associated with various diseases. The aim of the present study was to explore a possible influence of the 14-bp insertion/deletion polymorphism on osteosarcoma. METHODS: Genomic DNA was extracted from 75 formalin-fixed, paraffin-embedded tumor tissues derived from patients with conventional osteosarcoma (OSA) and 183 peripheral blood samples of healthy controls. Fifty-eight cases were South Korean patients with OSA and 17 cases were Argentine patients with OSA. The HLA-G 14-bp insertion/deletion polymorphism at exon 8 of the HLA-G locus was analyzed by polymerase chain reaction. RESULTS: There was a significantly different distribution profile for the 14-bp genotypes between the Korean OSA and Korean control groups. Specifically, there were more heterozygote 210 bp/224 bp genotypes in the Korean OSA group when compared to the Korean control group (62.1% vs 40.4%, p=0.002). CONCLUSIONS: The results suggest that HLA-G heterozygote patients may be more susceptible to OSA in the Korean population.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
DNA
/
Osteossarcoma
/
Éxons
/
Transplante de Órgãos
/
Transplantes
/
Antígenos HLA-G
/
Genótipo
/
Heterozigoto
/
Leucócitos
/
Complexo Principal de Histocompatibilidade
Limite:
Humans
Idioma:
En
Revista:
Korean Journal of Pathology
Ano de publicação:
2011
Tipo de documento:
Article