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Effects of Ranibizumab, Bevacizumab, and Aflibercept on Senescent Retinal Pigment Epithelial Cells
Korean Journal of Ophthalmology ; : 328-338, 2018.
Artigo em Inglês | WPRIM | ID: wpr-716255
ABSTRACT

PURPOSE:

Anti-vascular endothelial growth factor (VEGF) agents have been used for the last 10 years, but their safety profile, including cytotoxicity against various ocular cells such as retinal pigment epithelial (RPE) cells, remains a serious concern. Safety studies of VEGF agents conducted to date have primarily relied on healthy RPE cells. In this study, we assessed the safety of three anti-VEGF agents, namely, ranibizumab, bevacizumab, and aflibercept, on senescent RPE cells.

METHODS:

Senescent human induced pluripotent stem cell-derived RPE cells were generated by continuous replication and confirmed with senescence biomarkers. The viability, proliferation, protein expression, and phagocytosis of the senescent RPE cells were characterized 3 days after anti-VEGF treatment with clinical doses of ranibizumab, bevacizumab, or aflibercept.

RESULTS:

Clinical doses of ranibizumab, bevacizumab, or aflibercept did not decrease the viability or alter proliferation of senescent RPE cells. In addition, the anti-VEGF agents did not induce additional senescence, impair the protein expression of zonula occludens-1 and RPE65, or reduce the phagocytosis capacity of senescent RPE cells.

CONCLUSIONS:

Clinical dosages of ranibizumab, bevacizumab, or aflibercept do not induce significant cytotoxicity in senescent RPE cells.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fagocitose / Retinaldeído / Envelhecimento / Biomarcadores / Fatores de Crescimento Endotelial / Fator A de Crescimento do Endotélio Vascular / Células Epiteliais / Bevacizumab / Ranibizumab Limite: Humanos Idioma: Inglês Revista: Korean Journal of Ophthalmology Ano de publicação: 2018 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fagocitose / Retinaldeído / Envelhecimento / Biomarcadores / Fatores de Crescimento Endotelial / Fator A de Crescimento do Endotélio Vascular / Células Epiteliais / Bevacizumab / Ranibizumab Limite: Humanos Idioma: Inglês Revista: Korean Journal of Ophthalmology Ano de publicação: 2018 Tipo de documento: Artigo