Evaluation of Potency on Diphtheria and Tetanus Toxoid for Adult Vaccines by In Vivo Toxin Neutralization Assay Using National Reference Standards
Osong Public Health and Research Perspectives
;
(6): 278-282, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-717730
ABSTRACT
OBJECTIVES:
Vaccinations against diphtheria and tetanus are essential in providing immunity against these bacterial infections. The potency of diphtheria and tetanus toxoid vaccines can be measured using the in vivo toxin neutralization assay. The limit of potency of this assay was determined only for children. Therefore, we assessed the potency of adult vaccines using this assay to identify the feasibility of limit for adult vaccines.METHODS:
Fifteen lots of tetanus-reduced diphtheria and tetanus-diphtheria-acellular pertussis vaccines were used. In vivo toxin neutralization and lethal challenge assays were conducted on each vaccine to calculate the potencies of the toxoids. National reference standards for toxins and antitoxins were used for in vivo toxin neutralization assay.RESULTS:
All 15 lots satisfied the limits of potency for lethal challenge assay. The potency of diphtheria and tetanus toxoids exceeded 1 and 8 units/mL, respectively, for in vivo toxin neutralization assay.CONCLUSION:
Although additional studies are required for new assays and limits, the current level of potency for adult vaccines as determined by in vivo toxin neutralization assay, was demonstrated in this study. Such efforts to improve assays are expected to promote the development of diphtheria and tetanus vaccines for adults and to contribute to vaccine self-sufficiency.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tétano
/
Infecções Bacterianas
/
Toxoides
/
Antitoxinas
/
Toxoide Diftérico
/
Toxoide Tetânico
/
Vacinas
/
Coqueluche
/
Vacinação
/
Difteria
Limite:
Adulto
/
Criança
/
Humanos
Idioma:
Inglês
Revista:
Osong Public Health and Research Perspectives
Ano de publicação:
2018
Tipo de documento:
Artigo
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