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The Suppressive Effect of Butyrate and Bromopyruvate on Inflammatory Cytokine Production and Short Chain Fatty Acid Receptor Expression by Blood Mononuclear Cells in Patients with Behçet's Disease
Annals of Dermatology ; : 566-574, 2018.
Artigo em Inglês | WPRIM | ID: wpr-717766
ABSTRACT

BACKGROUND:

Controlling inflammation is a therapeutic goal of various autoimmune/autoinflammatory diseases including Behçet's disease (BD). The immunomodulatory effect of metabolites or metabolic analogs such as butyrate and 3-bromopyruvate has been observed in animal disease models.

OBJECTIVE:

We attempted to evaluate the effect of butyrate and 3-bromopyruvate on the inflammatory cytokine production by peripheral blood mononuclear cells (PBMCs) isolated from patients with mucocutaneous involvement of BD.

METHODS:

PBMCs isolated from 11 patients with BD and 10 healthy controls were stimulated with lipopolysaccharide in the presence of butyrate or 3-bromopyruvate. Butyrate receptor and cytokine messenger ribonucleic acid (mRNA) expression was analyzed by real-time reverse transcription polymerase chain reaction. Cytokine secretion was assessed by enzyme-linked immunosorbent assay. PBMCs survival was analyzed by flow cytometry.

RESULTS:

Bromopyruvate or butyrate treatment suppressed inflammatory cytokine production in PBMCs from all our subjects. Bromopyruvate also reduced PBMCs survival while butyrate did not. As the effect of butyrate was slightly greater in BD patients than in healthy controls, we analyzed butyrate receptor expression and found that lipopolysaccharide-induced free fatty acid receptor 2 mRNA level in PBMCs was higher in BD patients than in controls.

CONCLUSION:

We propose bromopyruvate and butyrate as supplementary therapeutic candidates to control inflammation in patients with BD.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Doenças Autoimunes / Butiratos / RNA / RNA Mensageiro / Ensaio de Imunoadsorção Enzimática / Reação em Cadeia da Polimerase / Transcrição Reversa / Modelos Animais de Doenças / Citometria de Fluxo / Glicólise Limite: Humanos Idioma: Inglês Revista: Annals of Dermatology Ano de publicação: 2018 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Doenças Autoimunes / Butiratos / RNA / RNA Mensageiro / Ensaio de Imunoadsorção Enzimática / Reação em Cadeia da Polimerase / Transcrição Reversa / Modelos Animais de Doenças / Citometria de Fluxo / Glicólise Limite: Humanos Idioma: Inglês Revista: Annals of Dermatology Ano de publicação: 2018 Tipo de documento: Artigo