Freezing and Washing of Red Blood Cells Using Haemonetics ACP 215 / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 291-300, 2018.
Artigo
em Coreano
| WPRIM
| ID: wpr-718426
ABSTRACT
BACKGROUND:
The use of a functionally closed system for the glycerolization and deglycerolization of red blood cells (RBCs) allows for prolonged post-thaw storage for more than 24 hours. The aim of this study was to assess glycerolization and deglycerolization processing for RBCs using a high glycerol method in the automated, closed system provided by Haemonetics ACP 215.METHODS:
Thirty-five packed RBCs were glycerolized using the ACP 215 to a final concentration of 40% (wt/vol). The units were either frozen as such (n=30) or excess glycerol was removed (n=5) before freezing. After storage at −80℃, the units were thawed, deglycerolized and resuspended in SAG-M. The frozen-thawed RBCs were stored at 4℃, and analyzed for their stability and in vitro quality.RESULTS:
No prefreeze excess glycerol removal units showed significantly less potassium leakage during post-thaw storage compared to the prefreeze excess glycerol removal units. All measurements of the stability and in vitro quality of thawed RBCs prepared from frozen RBCs without the prefreeze removal of excess glycerol during post-thaw storage at 4℃ for 7 days were acceptable to the American Blood Bank Association's standards and European standards.CONCLUSION:
RBCs frozen without prefreeze removal of excess glycerol and the ACP 215 simplifies cryopreservation procedure and increases the stability of frozen-thawed RBCs. This increases the practical applicability of cryopreserved RBCs in blood transfusion practice.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Potássio
/
Bancos de Sangue
/
Transfusão de Sangue
/
Técnicas In Vitro
/
Criopreservação
/
Eritrócitos
/
Congelamento
/
Glicerol
/
Métodos
Tipo de estudo:
Guia de Prática Clínica
Idioma:
Coreano
Revista:
Korean Journal of Blood Transfusion
Ano de publicação:
2018
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS