Association between Beta-lactam Antibiotic Resistance and Virulence Factors in AmpC Producing Clinical Strains of P. aeruginosa
Osong Public Health and Research Perspectives
;
(6): 325-333, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-718596
ABSTRACT
OBJECTIVES:
The purpose of this study was to determine the presence of IMP and OXA genes in clinical strains of Pseudomonas aeruginosa (P. aeruginosa) that are carriers of the ampC gene.METHODS:
In this study, 105 clinical isolates of P. aeruginosa were collected. Antibiotic resistance patterns were determined using the disk diffusion method. The strains carrying AmpC enzymes were characterized by a combination disk method. Multiplex-PCR was used to identify resistance and virulence genes, chi-square test was used to determine the relationship between variables.RESULTS:
Among 105 isolates of P. aeruginosa, the highest antibiotic resistance was to cefotaxime and aztreonam, and the least resistance was to colictin and ceftazidime. There were 49 isolates (46.66%) that showed an AmpC phenotype. In addition, the frequencies of the resistance genes were; OXA48 gene 85.2%, OXA199, 139 3.8%, OXA23 3.8%, OXA2 66.6%, OXA10 3.8%, OXA51 85.2% and OXA58 3.8%. The IMP27 gene was detected in 9 isolates (8.57%) and the IMP3.34 was detected in 11 isolates (10.47%). Other genes detected included; lasR (17.1%), lasB (18%) and lasA (26.6%). There was a significant relationship between virulence factors and the OX and IMP genes (p ≤ 0.05).CONCLUSION:
The relationship between antibiotic resistance and virulence factors observed in this study could play an important role in outbreaks associated with P. aeruginosa infections.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Pseudomonas aeruginosa
/
Virulência
/
Beta-Lactamases
/
Aztreonam
/
Resistência Microbiana a Medicamentos
/
Cefotaxima
/
Ceftazidima
/
Surtos de Doenças
/
Fatores de Virulência
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Osong Public Health and Research Perspectives
Ano de publicação:
2018
Tipo de documento:
Artigo
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