Selection of Unnecessary Urine Culture Specimens Using Sysmex UF-5000 Urine Flow Cytometer / 대한임상미생물학회지
Annals of Clinical Microbiology
;
: 75-79, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-718745
ABSTRACT
BACKGROUND:
Urine culture is one of the most frequently requested tests in microbiology. Automated urine analyzers yield much infection-related information. The Sysmex UF-5000 analyzer (Sysmex, Japan) is a new flow cytometry urine analyzer capable of quantifying urinary particles, including bacteria, WBCs, and yeast-like cells (YLCs) and can provide a Gram stainability flag. In this work, we evaluated how many unnecessary urine cultures could be screened out using the UF-5000.METHODS:
We compared the culture results of 126 urine samples among 453 requested urine cultures (from sources other than the Urology and Nephrology departments) with urinalysis results. Urine cultures were considered positive if bacterial or YLC growth was ≥104 CFUs/mL.RESULTS:
We used urinalysis cut-off values of 50/µL and 100/µL for bacteria and YLC, respectively. Forty eight of the 126 (38.1%, or 10.6% of 453 requested) cultures were below these cut-off values and did not contain any culture-positive samples.CONCLUSION:
Bacteria and YLC counts generated using the UF-5000 analyzer could be used to screen out negative cultures and reduce urine culture volume by ~10% without sacrificing detection of positive cultures.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bactérias
/
Infecções Urinárias
/
Urologia
/
Urinálise
/
Citometria de Fluxo
/
Nefrologia
Idioma:
Inglês
Revista:
Annals of Clinical Microbiology
Ano de publicação:
2018
Tipo de documento:
Artigo
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