Usefulness of Track-C (Total HCV Core Antigen) Assays in Anti-HCV Positive Patients / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 244-249, 2004.
Artigo
em Coreano
| WPRIM
| ID: wpr-71938
ABSTRACT
BACKGROUND:
Virologic diagnosis of hepatitis C virus (HCV) infection is based on the use of sero-logic assays detecting specific anti-HCV antibodies, and then definitive diagnosis is made by detecting HCV RNA. Recently, newly developed Track-C (total HCV core antigen) test using an enzyme immunoassay (EIA) can detect and quantify total HCV core antigen in the peripheral blood of HCV-infected patients. In this study, the usefulness of Track-C test for the detection of HCV viremia was investigated by comparing the results with those of the HCV RNA test.METHODS:
The study group consisted of 159 sera including 72 anti-HCV positive sera. The Track-C test was performed by enzyme immunoassay (Ortho Clinical Diagnostics, USA) with pretreatment for the dissociation of antigen-antibody complex. HCV RNA test was performed by HCV in house RT-nested PCR method. Results were calculated for the sensitivity, specificity and efficiency by comparing to each other.RESULTS:
The efficiency between HCV RNA and Track-C was 77.4% for the 72 anti-HCV positivesera. Comparing with the results of HCV RNA, Track-C assay showed the sensitivity and specificity of 56.0% and 96.4%, respectively. Track-C assay demonstrated a relatively good linearity (R2=0.9836) and reproducibility (CV=4.4%) at high concentrations.CONCLUSIONS:
Although the sensitivity of Track-C assay was not as high as that of HCV RT-PCR, a positive Track-C assay suggests the presence of HCV viremia, especially at higher concentrations. Track-C assay, therefore, may be used as a simple and supplementary test for HCV viremia and for follow-up monitoring.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Viremia
/
RNA
/
Reação em Cadeia da Polimerase
/
Seguimentos
/
Técnicas Imunoenzimáticas
/
Sensibilidade e Especificidade
/
Hepacivirus
/
Anticorpos Anti-Hepatite C
/
Diagnóstico
/
Complexo Antígeno-Anticorpo
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Coreano
Revista:
The Korean Journal of Laboratory Medicine
Ano de publicação:
2004
Tipo de documento:
Artigo
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