Effects of granulocyte-colony stimulating factor and the expression of its receptor on various malignant cells
Korean Journal of Hematology
;
: 219-224, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-720166
ABSTRACT
BACKGROUND:
Granulocyte-colony stimulating factor (G-CSF) is extensively used to improve neutrophil count during anti-cancer chemotherapy. We investigated the effects of G-CSF on several leukemic cell lines and screened for the expression of the G-CSF receptor (G-CSFR) in various malignant cells.METHODS:
We examined the effects of the most commonly used commercial forms of G-CSF (glycosylated lenograstim and nonglycosylated filgrastim) on various leukemic cell lines by flow cytometry. Moreover, we screened for the expression of G-CSFR mRNA in 38 solid tumor cell lines by using real-time PCR.RESULTS:
G-CSF stimulated proliferation (40-80% increase in proliferation in treated cells as compared to that in control cells) in 3 leukemic cell lines and induced differentiation of AML1/ETO+ leukemic cells. Among the 38 solid tumor cell lines, 5 cell lines (hepatoblastoma, 2 breast carcinoma, squamous cell carcinoma of the larynx, and melanoma cell lines) showed G-CSFR mRNA expression.CONCLUSION:
The results of the present study show that therapeutic G-CSF might stimulate the proliferation and differentiation of malignant cells with G-CSFR expression, suggesting that prescreening for G-CSFR expression in primary tumor cells may be necessary before using G-CSF for treatment.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes
/
Mama
/
RNA Mensageiro
/
Carcinoma de Células Escamosas
/
Linhagem Celular
/
Fator Estimulador de Colônias de Granulócitos
/
Receptores de Fator Estimulador de Colônias de Granulócitos
/
Linhagem Celular Tumoral
/
Citometria de Fluxo
/
Laringe
Idioma:
Inglês
Revista:
Korean Journal of Hematology
Ano de publicação:
2012
Tipo de documento:
Artigo
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