Elevated levels of activated and inactivated thrombin-activatable fibrinolysis inhibitor in patients with sepsis
Korean Journal of Hematology
;
: 264-268, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-720655
ABSTRACT
BACKGROUND:
In sepsis, large scale inflammatory responses can cause extensive collateral damage to the vasculature, because both coagulation and fibrinolysis are activated unevenly. Thrombin-activatable fibrinolysis inhibitor (TAFI) plays a role in modulating fibrinolysis. Since TAFI can be activated by both thrombin and plasmin, it is thought to be affected in sepsis. Hence, activated and inactivated TAFI (TAFIa/ai) may be used to monitor changes in sepsis.METHODS:
TAFIa/ai-specific in-house ELISA can detect only the TAFIa/ai form, because the ELISA capture agent is potato tuber carboxypeptidase inhibitor (PTCI), which has selective affinity towards only the TAFIa and TAFIai isoforms. TAFIa/ai levels in plasma from 25 patients with sepsis and 19 healthy volunteers were quantitated with the in-house ELISA.RESULTS:
We observed increased TAFIa/ai levels in samples from patients with sepsis (48.7+/-9.3 ng/mL) than in samples from healthy individuals (10.5+/-5.9 ng/mL). In contrast, no difference in total TAFI concentration was obtained between sepsis patients and healthy controls. The results suggest that TAFI zymogen was activated and that TAFIa/ai accumulated in sepsis.CONCLUSION:
The detection of TAFIa/ai in plasma could provide a useful and simple diagnostic tool for sepsis. Uneven activation of both coagulation and fibrinolysis in sepsis could be caused by the activation of TAFI zymogen and elevation of TAFIa/ai. TAFIa/ai could be a novel marker to monitor sepsis and other blood-related disturbances.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Compostos Organotiofosforados
/
Plasma
/
Solanum tuberosum
/
Ensaio de Imunoadsorção Enzimática
/
Trombina
/
Fibrinolisina
/
Sepse
/
Isoformas de Proteínas
/
Carboxipeptidase B2
/
Fibrinólise
Limite:
Humanos
Idioma:
Inglês
Revista:
Korean Journal of Hematology
Ano de publicação:
2010
Tipo de documento:
Artigo
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