Molecular Epidemiological Typing of Methicillin-Resistant Staphylococcus aureus Using Random Amplified Polymorphic DNA Analysis / 감염과화학요법
Infection and Chemotherapy
;
: 39-46, 2005.
Artigo
em Coreano
| WPRIM
| ID: wpr-721753
ABSTRACT
BACKGROUND:
The random amplified polymorphic DNA (RAPD) analysis was investigated to see if this method could be a useful tool for monitoring of epidemic outbreaks of methicillin-resistant Staphylococcus aureus (MRSA) among patients and healthcare workers (HCW) in the intensive care units (ICU).METHODS:
Thirty-eight MRSA strains were isolated from patients and HCW in Dong-A University Hospital ICU from October, 1998 to December, 1998 (10 patients and 8 HCW) and May, 2001 to July, 2001 (15 patients and 5 HCW). All strains were typed according to antimicrobial susceptibility and RAPD analysis patterns. mecA genes were detected using polymerase chain reaction (PCR).RESULTS:
Twenty one of 25 (84%) and 12 of 13 (92%) MRSA, isolated from patients and HCW, respectively, were mecA positive. mecA positive MRSA were classified into 18 different types by RAPD analysis.CONCLUSION:
DNA fingerprinting using RAPD analysis is a simple, effective, and rapid method for discriminating MRSA strains, and may be applicable in detecting outbreaks of S. aureus infections in the ICU.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
Reação em Cadeia da Polimerase
/
Surtos de Doenças
/
Impressões Digitais de DNA
/
Resistência a Meticilina
/
Atenção à Saúde
/
Staphylococcus aureus Resistente à Meticilina
/
Unidades de Terapia Intensiva
Tipo de estudo:
Ensaio Clínico Controlado
Limite:
Humanos
Idioma:
Coreano
Revista:
Infection and Chemotherapy
Ano de publicação:
2005
Tipo de documento:
Artigo
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