The Effects of Human Adipose Tissue Derived Mesenchymal Stem Cells on Degenerative Change of Disc in Rabbit Model
Journal of the Korean Academy of Rehabilitation Medicine
;
: 269-277, 2007.
Artigo
em Coreano
| WPRIM
| ID: wpr-723973
ABSTRACT
OBJECTIVE:
To determine whether transplanted human adipose tissue derived stem cells (hATSCs) can survive and increase the amount of proteoglycans in degenerated intervertebral disc.METHOD:
Lumbar disc degeneration was induced in thirty New Zealand white rabbits by injection of chondroitinase ABC(R). After 2 weeks, hATSCs were transplanted in degenerated disc in hATSCs group. Control group received phosphate buffered saline. The histologic grading and height of disc were measured at 2, 4, and 8 weeks after transplantation. The viability of donor cells was identified by using beta-Actin gene polymerase chain reaction (PCR).RESULTS:
4 and 8 weeks after hATSCs transplantation, the histologic grading showed significantly high score in hATSCs group (p<0.05), but the amount of proteoglycans was not significantly different between the two groups. The change of disc height was not significantly increased in hATSCs group. In the beta-Actin gene PCR analysis, positive signal in the hATSCs group was observed.CONCLUSION:
hATSCs transplantation may be useful in decelerating disc degeneration in experimental models and provide new hopes for treatment of degenerative disc disease in humans
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteoglicanas
/
Células-Tronco
/
Doadores de Tecidos
/
Tecido Adiposo
/
Reação em Cadeia da Polimerase
/
Actinas
/
Degeneração do Disco Intervertebral
/
Células-Tronco Mesenquimais
/
Esperança
/
Disco Intervertebral
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Academy of Rehabilitation Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo
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