Effects of Combined Treatments of Lithium and Valproate on the Phosphorylation of ERK1/2 and Transcriptional Activity of ELK1 and C-FOS in PC12 Cells
Journal of the Korean Society of Biological Psychiatry
;
: 159-165, 2013.
Artigo
em Coreano
| WPRIM
| ID: wpr-725002
ABSTRACT
OBJECTIVES:
Mechanisms of clinical synergistic effects, induced by co-treatments of lithium and valproate, are unclear. Extracellular signal-regulated kinase (ERK) has been suggested to play important roles in mechanisms of the action of mood stabilizers. In this study, effects of co-treatments of lithium and valproate on the ERK1/2 signal pathway and its down-stream transcription factors, ELK1 and C-FOS, were investigated in vitro.METHODS:
PC12 cells, human pheochromocytoma cells, were treated with lithium chloride (30 mM), valproate (1 mM) or lithium chloride + valproate. The phosphorylation of ERK1/2 was analyzed with immunoblot analysis. Transcriptional activities of ELK1 and C-FOS were analyzed with reporter gene assay.RESULTS:
Single treatment of lithium and valproate increased the phosphorylation of ERK and transcriptional activities of ELK1 and C-FOS, respectively. Combined treatments of lithium and valproate induced more robust increase in the phosphorylation of ERK1/2 and transcriptional activities of ELK1 and C-FOS, compared to those in response to single treatment of lithium or valproate.CONCLUSIONS:
Co-treatments of lithium and valproate induced synergistic increase in the phosphorylation of ERK1/2 and transcriptional activities of its down-stream transcription factors, ELK1 and C-FOS, compared to effects of single treatment. The findings might suggest potentiating effects of lithium and valproate augmentation treatment strategy.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Feocromocitoma
/
Fosforilação
/
Fosfotransferases
/
Fatores de Transcrição
/
Transdução de Sinais
/
Ácido Valproico
/
Células PC12
/
Cloreto de Lítio
/
Genes Reporter
/
Lítio
Limite:
Animais
/
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Society of Biological Psychiatry
Ano de publicação:
2013
Tipo de documento:
Artigo
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