The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Journal of Veterinary Science
; : 277-280, 2006.
Article
em En
| WPRIM
| ID: wpr-72554
Biblioteca responsável:
WPRO
ABSTRACT
Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently, B. hyodysenteriae and B. pilosicoli revealed different restriction patterns; however, the other three species shared the same pattern. These findings are not consistent with a prior report. Differences in 23S rRNA gene sequences, between two B. murdochii strains, 56-150 and 155-20, were observed. These results indicate that 23S rRNA PCR-RFLP could be used as an identification method for pathogenic Brachyspira spp. (B. hyodysenteriae and B. pilosicoli) as well as an epidemiological tool for characterizing spirochetes isolated from swine.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Filogenia
/
Spirochaetales
/
Infecções por Spirochaetales
/
Suínos
/
Doenças dos Suínos
/
Polimorfismo de Fragmento de Restrição
/
DNA Bacteriano
/
RNA Ribossômico 23S
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Reação em Cadeia da Polimerase
/
Disenteria Bacilar
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Journal of Veterinary Science
Ano de publicação:
2006
Tipo de documento:
Article