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Effect of Extremely Low Frequency Electromagnetic Fields (EMF) on Phospholipase Activity in the Cultured Cells
The Korean Journal of Physiology and Pharmacology ; : 427-433, 2010.
Artigo em Inglês | WPRIM | ID: wpr-727387
ABSTRACT
This study was conducted to investigate the effects of extremely low frequency electromagnetic fields (EMF) on signal pathway in plasma membrane of cultured cells (RAW 264.7 cells and RBL 2H3 cells), by measuring the activity of phospholipase A2 (PLA2), phospholipase C (PLC) and phospholipase D (PLD). The cells were exposed to the EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h. The basal and 0.5 microM melittin-induced arachidonic acid release was not affected by EMF in both cells. In cell-free PLA2 assay, we failed to observe the change of cPLA2 and sPLA2 activity. Also both PLC and PLD activities did not show any change in the two cell lines exposed to EMF. This study suggests that the exposure condition of EMF (60 Hz, 0.1 or 1 mT) which is 2.4 fold higher than the limit of occupational exposure does not induce phospholipases-associated signal pathway in RAW 264.7 cells and RBL 2H3 cells.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fosfolipases Tipo C / Fosfolipase D / Fosfolipases / Piridoxal / Transdução de Sinais / Linhagem Celular / Membrana Celular / Células Cultivadas / Exposição Ocupacional / Ácido Araquidônico Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 2010 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fosfolipases Tipo C / Fosfolipase D / Fosfolipases / Piridoxal / Transdução de Sinais / Linhagem Celular / Membrana Celular / Células Cultivadas / Exposição Ocupacional / Ácido Araquidônico Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 2010 Tipo de documento: Artigo