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Direct Corticosteroid Modulation of GABAergic Neurons in the Anterior Hypothalamic Area of GAD65-eGFP Mice
The Korean Journal of Physiology and Pharmacology ; : 163-169, 2011.
Artigo em Inglês | WPRIM | ID: wpr-727886
ABSTRACT
Corticosterone is known to modulate GABAergic synaptic transmission in the hypothalamic paraventricular nucleus. However, the underlying receptor mechanisms are largely unknown. In the anterior hypothalamic area (AHA), the sympathoinhibitory center that project GABAergic neurons onto the PVN, we examined the expression of glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) of GABAergic neurons using intact GAD65-eGFP transgenic mice, and the effects of corticosterone on the burst firing using adrenalectomized transgenic mice. GR or MR immunoreactivity was detected from the subpopulations of GABAergic neurons in the AHA. The AHA GABAergic neurons expressed mRNA of GR (42%), MR (38%) or both (8%). In addition, in brain slices incubated with corticosterone together with RU486 (MR-dominant group), the proportion of neurons showing a burst firing pattern was significantly higher than those in the slices incubated with vehicle, corticosterone, or corticosterone with spironolactone (GR-dominant group; 64 vs. 11~14%, p<0.01 by chi2-test). Taken together, the results show that the corticosteroid receptors are expressed on the GABAergic neurons in the AHA, and can mediate the corticosteroid-induced plasticity in the firing pattern of these neurons. This study newly provides the experimental evidence for the direct glucocorticoid modulation of GABAergic neurons in the AHA in the vicinity of the PVN.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Núcleo Hipotalâmico Paraventricular / Plásticos / Espironolactona / Encéfalo / RNA Mensageiro / Corticosterona / Camundongos Transgênicos / Receptores de Glucocorticoides / Receptores de Esteroides / Mifepristona Limite: Animais Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 2011 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Núcleo Hipotalâmico Paraventricular / Plásticos / Espironolactona / Encéfalo / RNA Mensageiro / Corticosterona / Camundongos Transgênicos / Receptores de Glucocorticoides / Receptores de Esteroides / Mifepristona Limite: Animais Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 2011 Tipo de documento: Artigo