Comparing the anti-inflammatory effect of nanoencapsulated lycopene and lycopene on RAW 264.7 macrophage cell line / 한국영양학회지
Journal of Nutrition and Health
;
: 459-467, 2015.
Artigo
em Coreano
| WPRIM
| ID: wpr-72790
ABSTRACT
PURPOSE:
We developed a method to load lycopene into maltodextrin and cyclodextrin in an attempt to overcome the poor bioavailability and improve the anti-inflammatory effect of this polyphenolMETHODS:
Nanosized lycopenes were encapsulated into biodegradable amphiphillic cyclodextrin and maltodextrin molecules prepared using a high pressure homogenizer at 15,000~25,000 psi. Cell damage was induced by lipopolysaccharides (LPS) in a mouse macrophage cell line, RAW 264.7. The cells were subjected to various doses of free lycopene (FL) and nanoencapsulated lycopene (NEL). RT-PCR was used to quantify the tumor necrosis factor (TNF-alpha), interleukin-1beta (IL-1beta), IL-6, inducible nitric oxide synthase (iNOS), and cyclooxigenase-2 (COX-2) mRNA levels, while ELISA was used to determine the protein levels of TNF-alpha, IL-1beta, and IL-6.RESULTS:
NEL significantly reduced the mRNA expression of IL-6 and IL-1beta at the highest dose, while not in cells treated with FL. In addition, NEL treatment caused a significant reduction in IL-6 and TNF-alpha protein levels, compared to cells treated with a similar dose of FL. In addition, mRNA expression of iNOS and COX-2 enzyme in the activated macrophages was more efficiently suppressed by NEL than by FL.CONCLUSION:
Overall, our results suggest that lycopene is a potential inflammation reducing agent and nanoencapsulation of lycopene can further improve its anti-inflammatory effect during tissue-damaging inflammatory conditions.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Ensaio de Imunoadsorção Enzimática
/
Disponibilidade Biológica
/
Linhagem Celular
/
Lipopolissacarídeos
/
Interleucina-6
/
Fator de Necrose Tumoral alfa
/
Óxido Nítrico Sintase Tipo II
/
Interleucina-1beta
/
Inflamação
Limite:
Animais
Idioma:
Coreano
Revista:
Journal of Nutrition and Health
Ano de publicação:
2015
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS