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Transduction of Tat-Superoxide Dismutase into Insulin-producing MIN6N Cells Reduces Streptozotocin-induced Cytotoxicity
The Korean Journal of Physiology and Pharmacology ; : 163-168, 2003.
Artigo em Inglês | WPRIM | ID: wpr-727908
ABSTRACT
The reactive oxygen species (ROS) are considered to be an important mediator in pancreatic beta cell destruction, thereby triggering the development of insulin-dependent diabetes mellitus. In the present study, HIV-1 Tat-mediated transduction of Cu, Zn-superoxide dismutase (SOD) was investigated to evaluate its protective potential against streptozotocin (STZ) -induced cytotoxicity in insulin-producing MIN6N cells. Tat-SOD fusion protein was successfully delivered into MIN6N cells in a dose-dependent manner and the transduced fusion protein was enzymatically active for 48 h. The STZ induced-cell destruction, superoxide anion radical production, and DNA fragmentation of MIN6N cells were significantly decreased in the cells pretreated with Tat-SOD for 1 h. Furthermore, the transduction of Tat-SOD increased Bcl-2 and heat shock protein 70 (hsp70) expressions in cells exposed to STZ, which might be partly responsible for the effect of Tat-SOD. These results suggest that an increased of free radical scavenging activity by transduction of Tat-SOD enhanced the tolerance of the cell against oxidative stress in STZ-treated MIN6N cells. Therefore, this Tat-SOD transduction technique may provide a new strategy to protect the pancreatic beta cell destruction in ROS-mediated diabetes.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Superóxido Dismutase / HIV-1 / Espécies Reativas de Oxigênio / Estreptozocina / Superóxidos / Estresse Oxidativo / Proteínas de Choque Térmico HSP70 / Diabetes Mellitus Tipo 1 / Células Secretoras de Insulina / Fragmentação do DNA Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Superóxido Dismutase / HIV-1 / Espécies Reativas de Oxigênio / Estreptozocina / Superóxidos / Estresse Oxidativo / Proteínas de Choque Térmico HSP70 / Diabetes Mellitus Tipo 1 / Células Secretoras de Insulina / Fragmentação do DNA Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 2003 Tipo de documento: Artigo