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Effect of adenosine on the release of (3H)-5-hydroxytryptamine during glucose/oxygen deprivation from rat hippocampal slices
The Korean Journal of Physiology and Pharmacology ; : 657-664, 1997.
Artigo em Inglês | WPRIM | ID: wpr-727966
ABSTRACT
The effects of adenosine, adenosine A1 receptor antagonist (DPCPX), or NMDA receptor antagonist (APV) on the spontaneous release of (3H)-5-hydroxytryptamine ((3H)-5-HT) during normoxic/normoglycemic or hypoxic/hypoglycemic period were studied in the rat hippocampal slices. The hippocampus was obtained from the rat brain and sliced 400 mum thickness with the tissue slicer. After 30 min's preincubation in the normal buffer, the slices were incubated for 30 min in a buffer containing (3H)-5-HT (0.1 muM, 74 muCi/8 ml) for uptake, and washed. To measure the release of (3H)-5-HT into the buffer, the incubation medium was drained off and refilled every ten minutes through sequence of 14 tubes. Induction of glucose/oxygen deprivation (GOD; medium depleting glucose and gassed with 95% N2/5% CO2) was done in 6th and 7th tube. The radioactivities in each buffer and the tissue were counted using liquid scintillation counter and the results were expressed as a percentage of the total radioactivities. When slices were exposed to GOD for 20 mins, the spontaneous release of (3H)-5-HT was markedly increased and this increase of (3H)-5-HT release was blocked by adenosine (10 muM) or DL-2-amino-5-phosphonovaleric acid (APV; 30 muM). Adenosine A1 receptor specific antagonist, 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) exacerbate GOD-induced increase of spontaneous release of (3H)-5-HT. These results suggest that Adenosine may play a role in the GOD-induced spontaneous release of (3H)-5-HT through adenosine A1 receptor activity.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Radioatividade / Contagem de Cintilação / Encéfalo / Adenosina / N-Metilaspartato / Receptor A1 de Adenosina / Glucose / Hipocampo Limite: Animais Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 1997 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Radioatividade / Contagem de Cintilação / Encéfalo / Adenosina / N-Metilaspartato / Receptor A1 de Adenosina / Glucose / Hipocampo Limite: Animais Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 1997 Tipo de documento: Artigo