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Development of Estimation Methods of Skin Oxidation and Evaluation of Anti-Oxidative Effects of Genistein in Topical Formulations
The Korean Journal of Physiology and Pharmacology ; : 205-209, 2012.
Artigo em Inglês | WPRIM | ID: wpr-728099
ABSTRACT
The objective of the present study was to establish the method of measurement of hydrogen peroxide and to estimate the anti-oxidative effect of genistein in the skin. UVB induced skin oxidation and anti-oxidative effect of genistein formulations were evaluated by determining levels of hydrogen peroxide. The mechanism involved in the determination of hydrogen peroxide is based on a color reaction between ferric ion (Fe3+) and xylenol orange, often called FOX assay and subsequent monitoring of absorbance values of the reactant at 540 nm. The reaction was to some extent pH-dependent and detection sensitivity was greatest at pH 1.75. Genistein liposomal gel demonstrated better anti-oxidative effect with regard to lowering hydrogen peroxide levels elevated by UVB irradiation compared to genistein-suspended gel. A linear relationship has been observed between anti-oxidative effect of genistein and drug deposition in the skin tissue. Genistein liposomal gel resulting in the localization of the drug in the deeper skin led to improved anti-oxidative effect compared to genistein gel. The suggested method for evaluation of oxidation of the skin can be used as a tool to screen effective anti-oxidative agents and their delivery systems acting on the skin.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fenóis / Pele / Sulfóxidos / Genisteína / Citrus sinensis / Concentração de Íons de Hidrogênio / Peróxido de Hidrogênio Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 2012 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fenóis / Pele / Sulfóxidos / Genisteína / Citrus sinensis / Concentração de Íons de Hidrogênio / Peróxido de Hidrogênio Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 2012 Tipo de documento: Artigo