The characterization of the increase of membrane conductance after depolarization in single rat adrenal chromaffin cells
The Korean Journal of Physiology and Pharmacology
;
: 95-100, 1998.
Artigo
em Inglês
| WPRIM
| ID: wpr-728155
ABSTRACT
The conductance change evoked by step depolarization was studied in primarily cultured rat adrenal chromaffin cells using patch-clamp and capacitance measurement techniques. When we applied a depolarizing pulse to a chromaffin cell, the inward calcium current was followed by an outward current and depolarization-induced exocytosis was accompanied by an increase in conductance trace. The slow inward tail current which has the same time course as the conductance change was observed in current recording. The activation of slow tail current was calcium-dependent. Reversal potentials agreed with Nernst equation assuming relative permeability of Cs+ to K+ is 0.095. The outward current and tail current were blocked by apamin (200 nM) and d-tubocurarine (2 mM). The conductance change was blocked by apamin and did not affect membrane capacitance recording. We confirmed that conductance change after depolarization comes from the activation of the SK channel and can be blocked by application of the SK channel blockers. Consequently, it is necessary to consider blocking of the SK channel during membrane capacitance recording.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Apamina
/
Permeabilidade
/
Tubocurarina
/
Cálcio
/
Técnicas de Patch-Clamp
/
Células Cromafins
/
Exocitose
/
Membranas
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
1998
Tipo de documento:
Artigo
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