Comparison of Ectopic Gene Expression Methods in Rat Neural Stem Cells
The Korean Journal of Physiology and Pharmacology
;
: 23-30, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-728176
ABSTRACT
Neural stem cells (NSCs) have the ability to proliferate and differentiate into various types of cells that compose the nervous system. To study functions of genes in stem cell biology, genes or siRNAs need to be transfected. However, it is difficult to transfect ectopic genes into NSCs. Thus to identify the suitable method to achieve high transfection efficiency, we compared lipid transfection, electroporation, nucleofection and retroviral transduction. Among the methods that we tested, we found that nucleofection and retroviral transduction showed significantly increased transfection efficiency. In addition, with retroviral transduction of Ngn2 that is known to induce neurogenesis in various types of cells, we observed facilitated final cell division in rat NSCs. These data suggest that nucleofection and retroviral transduction provide high efficiency of gene delivery system to study functions of genes in rat NSCs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Retroviridae
/
Células-Tronco
/
Biologia
/
Transfecção
/
Zidovudina
/
Expressão Gênica
/
Divisão Celular
/
Técnicas de Transferência de Genes
/
Eletroporação
/
RNA Interferente Pequeno
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
2013
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS