The transfection of caldesmon DNA into primary cultured rat aortic vascular smooth muscle
The Korean Journal of Physiology and Pharmacology
; : 597-603, 1999.
Article
em En
| WPRIM
| ID: wpr-728350
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WPRO
ABSTRACT
Caldesmon (CaD), one of microfilament-associated proteins, plays a key role in microfilament assembly in mitosis. We have investigated the effects of overexpression of the high molecular weight isoform of CaD (h-CaD) on the physiology of vascular smooth muscle cells (VSMCs). Rat aortic VSMCs were stably transfected with plasmids carrying a full length human h-CaD cDNA under control of cytomegalovirus promoter. The majority of the overexpressed h-CaD appears to be localized predominantly on cytoskeleton structures as determined by detergent lysis. The overexpression of h-CaD, however, does not decrease the level of endogenous low molecular weight isoform of CaD. h-CaD overexpressing VSMCs (h-CaD/VSMCs) show a decreased growth rate than that of vector-only transfected cells when determined by (3H)thymidine uptake and cell counting after fetal bovine serum (FBS) stimulation. h-CaD/VSMCs were smaller than vector-transfected cells by 18% in cell diameter. These data suggest that overexpression of h-CaD can inhibit the poliferation and the cell volume of VSMCs stimulated by growth factors and that the gene therapy with h-CaD may be helpful to prevent the conditions associated with hypertrophy and/or hyperplasia of VSMCs after arterial injuries.
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Assunto principal:
Fisiologia
/
Plasmídeos
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Citoesqueleto
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Citoesqueleto de Actina
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Proteínas de Ligação a Calmodulina
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DNA
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Transfecção
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Terapia Genética
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Contagem de Células
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DNA Complementar
Limite:
Animals
/
Humans
Idioma:
En
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
1999
Tipo de documento:
Article