Metformin Inhibits Isoproterenol-induced Cardiac Hypertrophy in Mice
The Korean Journal of Physiology and Pharmacology
;
: 377-384, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-728356
ABSTRACT
The present study examined whether metformin treatment prevents isoporterenol-induced cardiac hypertrophy in mice. Chronic subcutaneous infusion of isoproterenol (15 mg/kg/24 h) for 1 week using an osmotic minipump induced cardiac hypertrophy measured by the heart-to-body weight ratio and left ventricular posterior wall thickness. Cardiac hypertrophy was accompanied with increased interleukin-6 (IL-6), transforming growth factor (TGF)-beta, atrial natriuretic peptide (ANP), collagen I and III, and matrix metallopeptidase 2 (MMP-2). Coinfusion of metformin (150 mg/kg/24 h) with isoproterenol partially inhibited cardiac hypertrophy that was followed by reduced IL-6, TGF-beta, ANP, collagen I and III, and MMP-2. Chronic subcutaneous infusion of metformin did not increase AMP-activated protein kinase (AMPK) activity in heart, although acute intraperitoneal injection of metformin (10 mg/kg) increased AMPK activity. Isoproterenol increased nitrotyrosine levels and mRNA expression of antioxidant enzyme glutathione peroxidase and metformin treatment normalized these changes. These results suggest that metformin inhibits cardiac hypertrophy through attenuating oxidative stress.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tirosina
/
RNA Mensageiro
/
Fatores de Crescimento Transformadores
/
Fator de Crescimento Transformador beta
/
Colágeno
/
Interleucina-6
/
Fator Natriurético Atrial
/
Cardiomegalia
/
Estresse Oxidativo
/
Proteínas Quinases Ativadas por AMP
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
2010
Tipo de documento:
Artigo
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