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Acidic pH-activated Cl- Current and Intracellular Ca2+ Response in Human Keratinocytes
The Korean Journal of Physiology and Pharmacology ; : 177-183, 2008.
Artigo em Inglês | WPRIM | ID: wpr-728390
ABSTRACT
The layers of keratinocytes form an acid mantle on the surface of the skin. Herein, we investigated the effects of acidic pH on the membrane current and [Ca2+](c) of human primary keratinocytes from foreskins and human keratinocyte cell line (HaCaT). Acidic extracellular pH (pHe< or =5.5) activated outwardly rectifying Cl- current (I(Cl,pH)) with slow kinetics of voltage-dependent activation. I(Cl,pH) was potently inhibited by an anion channel blocker 4,4`-diisothiocyanostilbene-2,2`-disulphonic acid (DIDS, 73.5% inhibition at 1micrometer). I(Cl,pH) became more sensitive to pHe by raising temperature from 24degrees C to 37degrees C. HaCaT cells also expressed Ca2+ -activated Cl- current (I(Cl,Ca)), and the amplitude of I(Cl,Ca) was increased by relatively weak acidic pHe (7.0 and 6.8). Interestingly, the acidic pHe (5.0) also induced a sharp increase in the intracellular [Ca2+] (delta[Ca2+](acid)) of HaCaT cells. The delta[Ca2+](acid) was independent of extracellular Ca2+, and was abolished by the pretreatment with PLC inhibitor, U73122. In primary human keratinocytes, 5 out of 28 tested cells showed delta[Ca2+](acid). In summary, we found I(Cl,pH) and delta[Ca2+](acid) in human keratinocytes, and these ionic signals might have implication in pathophysiological responses and differentiation of epidermal keratinocytes.
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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Pirrolidinonas / Pele / Cinética / Queratinócitos / Linhagem Celular / Estrenos / Prepúcio do Pênis / Concentração de Íons de Hidrogênio / Membranas Limite: Humanos Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 2008 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Pirrolidinonas / Pele / Cinética / Queratinócitos / Linhagem Celular / Estrenos / Prepúcio do Pênis / Concentração de Íons de Hidrogênio / Membranas Limite: Humanos Idioma: Inglês Revista: The Korean Journal of Physiology and Pharmacology Ano de publicação: 2008 Tipo de documento: Artigo