Antioxidant effects of serotonin and L-DOPA on oxidative damages of brain synaptosomes
The Korean Journal of Physiology and Pharmacology
; : 147-155, 1999.
Article
em En
| WPRIM
| ID: wpr-728422
Biblioteca responsável:
WPRO
ABSTRACT
Antioxidant effects of serotonin and L-DOPA on neuronal tissues were examined by studying the oxidative damages of brain synaptosomal components. The study further explored the mechanism by which they exert protective actions. Serotonin and L-DOPA (1 muM to 1 mM) significantly inhibited lipid peroxidation of brain tissues by either Fe2+ and ascorbate or t-butyl hydroperoxide in a dose dependent fashion. Protective effect of serotonin on the peroxidative actions of both systems was greater than that of L-DOPA. Protein oxidation of synaptosomes caused by Fe2+ and ascorbate was attenuated by serotonin and L-DOPA. Protein oxidation more sensitively responded to L-DOPA rather than serotonin. Serotonin and L-DOPA (100 muM) decreased effectively the oxidation of synaptosomal sulfhydryl groups caused by Fe2+ and ascorbate. The production of hydroxyl radical caused by either Fe3+, EDTA, H2O2 and ascorbate or xanthine and xanthine oxidase was significantly decreased by serotonin and L-DOPA (1 mM). Equal concentrations of serotonin and L-DOPA restored synaptosomal Ca2+ uptake decreased by Fe2+ and ascorbate, which is responsible for SOD and catalase. Protective effects of serotonin and L-DOPA on brain synaptosomes may be attributed to their removing action on reactive oxidants, hydroxyl radicals and probably iron-oxygen complex, without chelating action on iron.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Sinaptossomos
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Xantina Oxidase
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Encéfalo
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Peroxidação de Lipídeos
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Catalase
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Levodopa
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Serotonina
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Oxidantes
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Ácido Edético
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Radical Hidroxila
Idioma:
En
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
1999
Tipo de documento:
Article