Multiple Regulation of Roundabout (Robo) Phosphorylation in a Heterologous Cell System
The Korean Journal of Physiology and Pharmacology
;
: 111-115, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-728494
ABSTRACT
Roundabout (Robo) is the transmembrane receptor for slit, the neuronal guidance molecule. In this study, the tyrosine phosphorylation of Robo was observed in Robo-transfected human embryonic kidney cells and developing rat brains, and found to be increased by the treatment with protein kinase A activator, forskolin. In contrast, protein kinase C activation by phorbol-12-myristate-13-acetate decreased the phosphorylation of Robo. Intracellular calcium was required for the tyrosine phosphorylation. Furthermore, the transfection of an Eph receptor tyrosine kinase dramatically enhanced the tyrosine phosphorylation. These findings indicate that the tyrosine phosphorylation of Robo is regulated by multiple mechanisms, and that Eph receptor kinases may play a role in the regulation of tyrosine phosphorylation of Robo in the rat brain.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosforilação
/
Fosfotransferases
/
Proteínas Quinases
/
Tirosina
/
Encéfalo
/
Colforsina
/
Proteína Quinase C
/
Transfecção
/
Cálcio
/
Proteínas Quinases Dependentes de AMP Cíclico
Tipo de estudo:
Guia de Prática Clínica
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
2004
Tipo de documento:
Artigo
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