High glucose and palmitate increases bone morphogenic protein 4 expression in human endothelial cells
The Korean Journal of Physiology and Pharmacology
;
: 169-175, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-728538
ABSTRACT
Here, we investigated whether hyperglycemia and/or free fatty acids (palmitate, PAL) aff ect the expression level of bone morphogenic protein 4 (BMP4), a proatherogenic marker, in endothelial cells and the potential role of BMP4 in diabetic vascular complications. To measure BMP4 expression, human umbilical vein endothelial cells (HUVECs) were exposed to high glucose concentrations and/or PAL for 24 or 72 h, and the effects of these treatments on the expression levels of adhesion molecules and reactive oxygen species (ROS) were examined. BMP4 loss-of-function status was achieved via transfection of a BMP4-specific siRNA. High glucose levels increased BMP4 expression in HUVECs in a dose-dependent manner. PAL potentiated such expression. The levels of adhesion molecules and ROS production increased upon treatment with high glucose and/or PAL, but this eff ect was negated when BMP4 was knocked down via siRNA. Signaling of BMP4, a proinflammatory and pro-atherogenic cytokine marker, was increased by hyperglycemia and PAL. BMP4 induced the expression of infl ammatory adhesion molecules and ROS production. Our work suggests that BMP4 plays a role in atherogenesis induced by high glucose levels and/or PAL.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transfecção
/
Espécies Reativas de Oxigênio
/
RNA Interferente Pequeno
/
Células Endoteliais
/
Diabetes Mellitus
/
Angiopatias Diabéticas
/
Aterosclerose
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Ácidos Graxos não Esterificados
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Células Endoteliais da Veia Umbilical Humana
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Glucose
Limite:
Humanos
Idioma:
Inglês
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
2016
Tipo de documento:
Artigo
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