Functional characteristics of neutral amino acid transporter in opossum kidney (OK) cells
The Korean Journal of Physiology and Pharmacology
;
: 185-193, 1997.
Artigo
em Inglês
| WPRIM
| ID: wpr-728634
ABSTRACT
The characteristics of Na+/-dependent cycloleucine uptake was investigated in OK cells with regard to substrate specificity and regulation by protein kinase C (PKC). Inhibition studies with different synthetic and natural amino acids showed a broad spectrum affinity to neutral amino acids regardless of their different side chains including branched or aromatic, indicating that the Na+/-dependent cycloleucine uptake in OK cells is mediated by System B-o or System B degree -like transporter rather than the classical System A or ASC. Phorbol 12-myristate 13-acetate (PMA) and phorbol 12,13-dibutyrate, but not 4 alpha-PMA elicited a time-dependent biphasic stimulation of Na+/-dependent cycloleucine uptake, which produced early transient peak at 30 min and late sustained peak at 180 min. Both the early and late stimulations by PMA were due to an increase in Vmax and not due to a change in Km. PKC inhibitors blocked both the early and late stimulation by PMA, while protein synthesis inhibitors blocked the late stimulation only. These results suggest the existence and regulation by PKC of System B degree or System B degree -like broad spectrum transport system for neutral amino acids in OK cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Gambás
/
Especificidade por Substrato
/
Proteína Quinase C
/
Inibidores da Síntese de Proteínas
/
Dibutirato de 12,13-Forbol
/
Aminoácidos Neutros
/
Cicloleucina
/
Sistemas de Transporte de Aminoácidos
/
Aminoácidos
/
Rim
Idioma:
Inglês
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
1997
Tipo de documento:
Artigo
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