Effect of kainic acid on the phosphorylation of mitogen activated protein kinases in rat hippocampus
The Korean Journal of Physiology and Pharmacology
;
: 451-456, 2001.
Artigo
em Inglês
| WPRIM
| ID: wpr-728786
ABSTRACT
In rat hippocampus, kainic acid (KA; 10 mg/kg; i.p.) increased the phosphorylated forms of ERK1/2 (p-ERK1/2) and Jun kinase1 (p-JNK1), but not p-JNK2 and p38 (p-p38). The preadministration with cycloheximide (CHX; 5 mg/kg; i.p.) inhibited KA-induced increase of p-JNK1, but not p-ERK1/2. Surprisingly, the phosphorylated upstream MAP kinase kinases (p-MKKs) were not correlated with their downstream MAP kinases. The basal p-MKK1/2 levels were completely abolished by KA, which were reversed by CHX. In addition, p-MKK4 and p-MKK3/6 levels were enhanced by CHX alone, but were attenuated by KA. Thus, our results showed that KA increased the p-ERK and p-JNK levels in rat hippocampus, which were not parallel with their classical upstreamal kinases.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosforilação
/
Fosfotransferases
/
Quinases de Proteína Quinase Ativadas por Mitógeno
/
Proteínas Quinases Ativadas por Mitógeno
/
Cicloeximida
/
Hipocampo
/
Ácido Caínico
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
2001
Tipo de documento:
Artigo
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