Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes
Mycobiology
;
: 104-107, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-729266
ABSTRACT
Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores. Transformation or gene tagging with Agrobacterium T-DNA based vector should be useful for wide ranges of genetic or molecular biological studies of the mushroom.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
Genoma Fúngico
/
Agaricales
/
Carpóforos
/
Flammulina
/
Agrobacterium
/
Brânquias
Limite:
Animais
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS