Comparison of RAPD, AFLP, and EF-1alpha Sequences for the Phylogenetic Analysis of Fusarium oxysporum and Its formae speciales in Korea
Mycobiology
;
: 45-55, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-729274
ABSTRACT
Although Fursarium oxysporum causes diseases in economically important plant hosts, identification of F. oxysporum formae speciales has been difficult due to confusing phenotypic classification systems. To resolve these complexity, we evaluated genetic relationship of nine formae speciales of F. oxysporum with random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and translation elongation factor-1 alpha (EF-1alpha) gene. In addition, the correlation between mycotoxin content of fusaric acid and isolates based on molecular marker data was evaluated using the modified Mantel's test. According to these result, these fusaric acid-producing strains could not identify clearly, and independent of geographic locations and host specificities. However, in the identification of F. oxysporum formae speciales, especially, AFLP analysis showed a higher discriminatory power than that of a the RAPD and EF-1alpha analyses, all three techniques were able to detect genetic variability among F. oxysporum formae speciales in this study.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas
/
DNA
/
Ácido Fusárico
/
Classificação
/
Fator 1 de Elongação de Peptídeos
/
Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
/
Especificidade de Hospedeiro
/
Fusarium
/
Localizações Geográficas
/
Coreia (Geográfico)
Tipo de estudo:
Estudo prognóstico
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2006
Tipo de documento:
Artigo
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