Effects of Age on in vitro Maturation and Fertilization of Immature Oocytes from Stimulated Cycles in Human IVF-ET Program / 대한불임학회지
Korean Journal of Fertility and Sterility
;
: 331-336, 2005.
Artigo
em Coreano
| WPRIM
| ID: wpr-72940
ABSTRACT
OBJECTIVE:
To investigate the effects of female age on in vitro maturation and fertilization of immature oocytes from controlled ovarian hyperstimulation (COH) in human IVF-ET program.METHOD:
A total of 96 immature oocytes (GV & metaphase I) obtained from 40 cycles of IVF-ET (29 patients). The mean age of female patients was 31.8+/-3.1 years. Ovulation was triggered by urinary or recombinant hCG. Immature oocytes were cultured with YS medium containing 30% of patients' human follicular fluids, LH (1 IU/mL), FSH (1 IU/mL) and EGF (10 ng/mL), and then matured oocytes were fertilized by ICSI. In vitro maturation and fertilization of immature oocytes were analyzed according to age of female ( or = 34 years).RESULTS:
The maturation rate was similar between two groups (68% vs 64%). The fertilization rate of in?vitro-matured oocytes was higher in patients < 34 years old, but there was no statistical significance (64% vs 50%, p=0.347). The fertilization rate of in-vitro-matured oocytes was significantly lower compared with those of in-vivo-matured oocytes in both age groups (64% vs 79%, p=0.035, 50% vs 86%, p=0.007).CONCLUSION:
In older female group, fertilization rate of in-vitro-matured oocytes seems to be decreased. Further investigations should be warranted to increase fertilization potential of in-vitro-matured oocytes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oócitos
/
Ovulação
/
Líquido Folicular
/
Injeções de Esperma Intracitoplásmicas
/
Fator de Crescimento Epidérmico
/
Fertilização
/
Metáfase
Limite:
Adulto
/
Feminino
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Fertility and Sterility
Ano de publicação:
2005
Tipo de documento:
Artigo
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