Generation and Evaluation of High beta-Glucan Producing Mutant Strains of Sparassis crispa
Mycobiology
;
: 159-163, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-729419
ABSTRACT
A chemical mutagenesis technique was employed for development of mutant strains of Sparassis crispa targeting the shortened cultivation time and the high beta-glucan content. The homogenized mycelial fragments of S. crispa IUM4010 strain were treated with 0.2 vol% methyl methanesulfonate, an alkylating agent, yielding 199 mutant strains. Subsequent screening in terms of growth and beta-glucan content yielded two mutant strains, B4 and S7. Both mutants exhibited a significant increase in beta-glucan productivity by producing 0.254 and 0.236 mg soluble beta-glucan/mg dry cell weight for the B4 and S7 strains, respectively, whereas the wild type strain produced 0.102 mg soluble beta-glucan/mg dry cell weight. The results demonstrate the usefulness of chemical mutagenesis for generation of mutant mushroom strains.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Entorses e Distensões
/
Programas de Rastreamento
/
Mutagênese
/
Mesilatos
/
Agaricales
/
Eficiência
/
Metanossulfonato de Metila
Tipo de estudo:
Estudo de rastreamento
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2013
Tipo de documento:
Artigo
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