Production and Characterization of a New alpha-Glucosidase Inhibitory Peptide from Aspergillus oryzae N159-1
Mycobiology
;
: 149-154, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-729421
ABSTRACT
An alpha-glucosidase inhibitor was developed from Aspergillus oryzae N159-1, which was screened from traditional fermented Korean foods. The intracellular concentration of the inhibitor reached its highest level when the fungus was cultured in tryptic soy broth medium at 27degrees C for five days. The inhibitor was purified using a series of purification steps involving ultrafiltration, Sephadex G-25 gel permeation chromatography, strong cation exchange solid phase extraction, reverse-phase high performance liquid chromatography, and size exclusion chromatography. The final yield of the purification was 1.9%. Results of the liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis indicated that the purified alpha-glucosidase inhibitor was a tri-peptide, Pro-Phe-Pro, with the molecular weight of 360.1 Da. The IC50 value of the peptide against alpha-glucosidase activity was 3.1 mg/mL. Using Lineweaver-Burk plot analysis, the inhibition pattern indicated that the inhibitor acts as a mixed type inhibitor.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Hidrolisados de Proteína
/
Aspergillus
/
Aspergillus oryzae
/
Espectrometria de Massas
/
Ultrafiltração
/
Caseínas
/
Cromatografia em Gel
/
Cromatografia Líquida
/
Dextranos
/
Concentração Inibidora 50
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2013
Tipo de documento:
Artigo
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