Development of Specific Primer for Tricholoma matsutake
Mycobiology
;
: 317-319, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-729424
ABSTRACT
In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'-GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Reação em Cadeia da Polimerase
/
Tricholoma
/
Coreia (Geográfico)
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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