Fungal Strain Improvement for Cellulase Production Using Repeated and Sequential Mutagenesis
Mycobiology
;
: 267-271, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-729434
ABSTRACT
A fungal strain producing a high level of cellulase was selected from 320 fungal isolates and identified as Aspergillus sp. This strain was further improved for cellulase production by sequential treatments by two repeated rounds of gamma-irradiation of Co60, ultraviolet treatment and four repeated rounds of treatment with N-methyl-N'-nitro-N-nitrosoguanidine. The best mutant strain, Aspergillus sp. XTG-4, was selected after screening and the activities of carboxymethyl cellulase, filter paper cellulase and beta-glucosidase of the cellulase were improved by 2.03-, 3.20-, and 1.80-fold, respectively, when compared to the wild type strain. After being subcultured 19 times, the enzyme production of the mutant Aspergillus sp. XTG-4s was stable.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Aspergillus
/
Entorses e Distensões
/
Celulase
/
Programas de Rastreamento
/
Mutagênese
/
Beta-Glucosidase
/
Metilnitronitrosoguanidina
Tipo de estudo:
Estudo de rastreamento
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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