An Efficient Method to Prepare PCR Cloning Vectors
Mycobiology
;
: 240-242, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-729439
ABSTRACT
An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by further digestion of the small fragment produced by XcmI digestion with additional enzymes, and purification with PCR purification kits. Using this procedure, PCR cloning vectors with high ligation efficiencies and low blue or false-positive colonies were obtained.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Reação em Cadeia da Polimerase
/
Células Clonais
/
Clonagem de Organismos
/
Digestão
/
Vetores Genéticos
/
Ligadura
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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