Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green
Mycobiology
; : 258-262, 2012.
Article
em En
| WPRIM
| ID: wpr-729695
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WPRO
ABSTRACT
cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H2O2 over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H2O2 improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 microM within 90 min.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Compostos Organometálicos
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Oxirredutases
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Peroxidases
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Pichia
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Corantes de Rosanilina
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DNA Complementar
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Peroxidase
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Phanerochaete
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Oxirredutases do Álcool
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Glioxal
Idioma:
En
Revista:
Mycobiology
Ano de publicação:
2012
Tipo de documento:
Article