Depletion of epsilon-COP in the COPI Vesicular Coat Reduces Cleistothecium Production in Aspergillus nidulans
Mycobiology
;
: 31-36, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-729750
ABSTRACT
We have previously isolated epsilon-COP, the alpha-COP interactor in COPI of Aspergillus nidulans, by yeast two-hybrid screening. To understand the function of epsilon-COP, the aneA+ gene for epsilon-COP/AneA was deleted by homologous recombination using a gene-specific disruption cassette. Deletion of the epsilon-COP gene showed no detectable changes in vegetative growth or asexual development, but resulted in decrease in the production of the fruiting body, cleistothecium, under conditions favorable for sexual development. Unlike in the budding yeast Saccharomyces cerevisiae, in A. nidulans, over-expression of epsilon-COP did not rescue the thermo-sensitive growth defect of the alpha-COP mutant at 42degrees C. Together, these data show that epsilon-COP is not essential for viability, but it plays a role in fruiting body formation in A. nidulans.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Aspergillus nidulans
/
Saccharomyces cerevisiae
/
Leveduras
/
Programas de Rastreamento
/
Proteína Coatomer
/
Desenvolvimento Sexual
/
Saccharomycetales
/
Recombinação Homóloga
/
Frutas
Tipo de estudo:
Estudo de rastreamento
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2015
Tipo de documento:
Artigo
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