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Preliminary Characterization of Keratinolytic Enzyme of Aspergillus flavus K-03 and Its Potential in Biodegradation of Keratin Wastes
Mycobiology ; : 209-213, 2003.
Artigo em Inglês | WPRIM | ID: wpr-729806
ABSTRACT
Aspergillus flavus K-03 isolated from poultry forming soil in Korea was studied for its ability to produce extracellular proteases on basal medium containing 2% (w/v) chicken feathers. The fungus was observed to be a potent producer of such enzymes. Keratinolytic enzyme secretion was the best at 15 days of incubation period at pH 9 and temperature 40degrees C. No relationship existed between the enzyme yield and increase of biomass. Enzyme production was suppressed by exogenous sugars in descending order arabinose>maltose>mannose>fructose. But glucose did not influence the enzyme activity. The keratinolytic enzyme released by the fungus demonstrated the ability to decompose keratin substrates as chicken feather when exogenous glucose was present. The keratinolytic activity was inhibited by HgCl2 and serine-protease inhibitors such as phenymethylsulfonyl fluoride (100%), chymostain (88%), crystalline soybean trypsin inhibtor (80%), antipain (45%) and aprotinin (40%), and was not by cystein-protease and aspartyl-protease inhibitors. The enzyme activity is only partially inhibited by metallo-protease inhibitor. Thus, the enzyme secreted by A. flavus K-03 belongs to the alkaline serine-type protease.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Peptídeo Hidrolases / Aves Domésticas / Aspergillus / Aspergillus flavus / Solo / Glycine max / Carboidratos / Tripsina / Galinhas / Aprotinina Limite: Animais País/Região como assunto: Ásia Idioma: Inglês Revista: Mycobiology Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Peptídeo Hidrolases / Aves Domésticas / Aspergillus / Aspergillus flavus / Solo / Glycine max / Carboidratos / Tripsina / Galinhas / Aprotinina Limite: Animais País/Região como assunto: Ásia Idioma: Inglês Revista: Mycobiology Ano de publicação: 2003 Tipo de documento: Artigo