In Silico Sequence Analysis Reveals New Characteristics of Fungal NADPH Oxidase Genes
Mycobiology
;
: 241-248, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-729881
ABSTRACT
NADPH oxidases (Noxes), transmembrane proteins found in most eukaryotic species, generate reactive oxygen species and are thereby involved in essential biological processes. However, the fact that genes encoding ferric reductases and ferric-chelate reductases share high sequence similarities and domains with Nox genes represents a challenge for bioinformatic approaches used to identify Nox-encoding genes. Further, most studies on fungal Nox genes have focused mainly on functionality, rather than sequence properties, and consequently clear differentiation among the various Nox isoforms has not been achieved. We conducted an extensive sequence analysis to identify putative Nox genes among 34 eukaryotes, including 28 fungal genomes and one Oomycota genome. Analyses were performed with respect to phylogeny, transmembrane helices, di-histidine distance and glycosylation. Our analyses indicate that the sequence properties of fungal Nox genes are different from those of human and plant Nox genes, thus providing novel insight that will enable more accurate identification and characterization of fungal Nox genes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oomicetos
/
Oxirredutases
/
Filogenia
/
Plantas
/
Fenômenos Biológicos
/
Simulação por Computador
/
Glicosilação
/
Genoma Fúngico
/
Genoma
/
Análise de Sequência
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2014
Tipo de documento:
Artigo
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