Proposal of a New Estimation Method of Colonization Rate of Arbuscular Mycorrhizal Fungi in the Roots of Chengiopanax sciadophylloides
Mycobiology
;
: 15-19, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-729894
ABSTRACT
This study proposed a rapid method to quantify the colonization rate of arbuscular mycorrhizal fungi (AMF) in plant roots. The method involved the use of an image analysis software (WinRHIZO Pro). The colonization rate is defined as the ratio of the fungal body to the plant root area in a micrograph. Three seedlings of Chengiopanax sciadophylloides, a woody species that accumulates radiocesium, were collected from a secondary forest in the Yamakiya district of Kawamata, Fukushima Prefecture during May–September 2014. The colonization of AMF structures was examined under a light microscope, and the percentage of colonization was determined using the WinRHIZO method. The superiority of the new method was verified by comparing with a modified grid-line intersect method. The colonization of AMF was confirmed in all the seedlings, and a significant coefficient of determination (R² = 0.94) was found with both the methods. The results suggested that the WinRHIZO method is reliable for estimating the colonization of AMF in C. sciadophylloides.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Florestas
/
Colo
/
Raízes de Plantas
/
Plântula
/
Fungos
/
Métodos
/
Microscopia
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2017
Tipo de documento:
Artigo
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