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Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses
Mycobiology ; : 338-342, 2016.
Artigo em Inglês | WPRIM | ID: wpr-729897
ABSTRACT
The culture filtrate of Lentinula edodes shows potent antimicrobial activity against the plant pathogenic bacteria Ralstonia solanacearum. Bioassay-guided fractionation was conducted using Diaion HP-20 column chromatography, and the insoluble active compound was not adsorbed on the resin. Further fractionation by high-performance liquid chromatography (HPLC) suggested that the active compounds were organic acids. Nine organic acids were detected in the culture filtrate of L. edodes; oxalic acid was the major component and exhibited antibacterial activity against nine different phytopathogenic bacteria. Quantitative analysis by HPLC revealed that the content of oxalic acid was higher in the water extract from spent mushroom substrate than in liquid culture. This suggests that the water extract of spent L. edodes substrate is an eco-friendly control agent for plant diseases.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Doenças das Plantas / Plantas / Bactérias / Água / Cromatografia / Cromatografia Líquida de Alta Pressão / Cromatografia Líquida / Lentinula / Ácido Oxálico / Cogumelos Shiitake Tipo de estudo: Pesquisa qualitativa Idioma: Inglês Revista: Mycobiology Ano de publicação: 2016 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Doenças das Plantas / Plantas / Bactérias / Água / Cromatografia / Cromatografia Líquida de Alta Pressão / Cromatografia Líquida / Lentinula / Ácido Oxálico / Cogumelos Shiitake Tipo de estudo: Pesquisa qualitativa Idioma: Inglês Revista: Mycobiology Ano de publicação: 2016 Tipo de documento: Artigo