Production of Blastospore of Entomopathogenic Beauveria bassiana in a Submerged Batch Culture
Mycobiology
;
: 218-224, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-729924
ABSTRACT
The principal objective of this study was to determine the optimal liquid culture conditions in shake flasks for maximal sporulation of Beauveria bassiana. The optimal initial pH for the spore production of B. bassiana using Potato Dextrose Broth was 5.2. The screening in shake flasks of carbon and nitrogen sources resulted in the identification of an optimal medium based on 3% sucrose and 1% casamino acid, with a C N ratio of 22 4. Using this medium, a production level of 5.65 x 107 spores per ml was obtained after 5 days of culture. Using 3% corn meal, 2% corn steep powder, and 2% rice bran, the maximum spore concentration of 8.54 x 108/ml was achieved 8 days after inoculation at 25degrees C in a rotary shaking incubator operated at 200 rpm. This represents a yield gain of approximately 2.89 times that of pre-optimization.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Esporos
/
Sacarose
/
Solanum tuberosum
/
Carbono
/
Programas de Rastreamento
/
Zea mays
/
Beauveria
/
Técnicas de Cultura Celular por Lotes
/
Refeições
/
Glucose
Tipo de estudo:
Estudo de rastreamento
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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