ITS Primers with Enhanced Specificity to Detect the Ectomycorrhizal Fungi in the Roots of Wood Plants
Mycobiology
;
: 23-31, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-729983
ABSTRACT
With universal primer ITS1-F, the specific DHJ2 primer was developed to detect the Ectomycorrhizal (ECM) root tips in soil and to identify the species of ECM fungi, as based on DNA sequences of rDNA stored in GeneBank of NCBI. This primer was designed with the common sites of rDNA of Amanita and Boletus, and was also designed with several DNA programs provided by NCBI. The DNA fragments synthesized by PCR were calculated to be 1,000 to 1,200 bps of DNA located to 18s to 28s rDNA to contain two variable sites of ITS, indicating much diversities for specific species or ecotypes of ECM fungi. The primer DHJ2 reacted with the genomic DNA's extracted from the tissues of basidiocarp at the rate of 73 of 80 fungi collected produced single bands with a 1,100 bps length. The DNA fragment synthesized with the genomic DNA that extracted from eight ECM tips of Pinus densiflora was confirmed and analysized to the rDNAs of ECM in full sequences, and informed to be a ECM fungal species in the forest.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Solo
/
Madeira
/
DNA
/
DNA Ribossômico
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Sensibilidade e Especificidade
/
Meristema
/
Pinus
/
Carpóforos
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2003
Tipo de documento:
Artigo
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