Isolation of Fungal Pathogens to an Edible Mushroom, Pleurotus eryngii, and Development of Specific ITS Primers
Mycobiology
;
: 252-255, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-730032
ABSTRACT
Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four strains of pathogenic fungi, including Trichoderma koningiopsis DC3, Phomopsis sp. MP4, Mucor circinelloides MP5, and Cladosporium bruhnei MP6, were isolated from the bottle culture of diseased P. eryngii. A species-specific primer set was designed for each fungus from the ITS1-5.8S rDNA-ITS2 sequences. PCR using the ITS primer set yielded a unique DNA band for each fungus without any cross-reaction, proving the validity of our method in detection of mushroom fungal pathogens.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Trichoderma
/
DNA
/
Reação em Cadeia da Polimerase
/
Cladosporium
/
Pleurotus
/
Agaricales
/
Fungos
/
Mucor
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2013
Tipo de documento:
Artigo
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