Production of the Acetylcholinesterase Inhibitor from Yarrowia lipolytica S-3
Mycobiology
;
: 102-105, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-730099
ABSTRACT
The acetylcholinesterase (AChE) inhibitor of Yarrowia lipolytica S-3 was maximally produced when it was incubated at 30degrees C for 36 h in an optimal medium containing 1% yeast extract, 2% peptone and 2% glucose, with an initial pH 6.0. The final AChE inhibitory activity under these conditions was an IC50 value of 64 mg/ml. After partial purification of the AChE inhibitor by means of systematic solvent extraction, the final IC50 value of the partially purified AChE inhibitor was 0.75 mg/ml. We prepared a test product by using the partially purified AChE inhibitor and then determined its stability for the development of a new antidementia commercial product. The test product was stable at room temperature for 15 weeks.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peptonas
/
Acetilcolinesterase
/
Leveduras
/
Concentração Inibidora 50
/
Yarrowia
/
Glucose
/
Concentração de Íons de Hidrogênio
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2008
Tipo de documento:
Artigo
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