Effects of Arsenite Exposure on the Expression Level of CaMKⅡin the Hippocampus of Offspring Mice / 沈阳医学院学报

ABSTRACT

Objective:To investigate the impairment mechanism of learning and memory ability induced by arsenite through studying the effects of arsenite on the expression level of CaMKⅡ in the hippocampus of offspring mice at different developmental stages. Methods:The model of offspring mice exposed to arsenite (0,15,30 and 60 mg/L NaAsO2) was set up. In postnatal day (PND) 10,20 and 40,the expression levels of CaMKⅡαand CaMKⅡβmRNA in the hippocampus were measured by real-time RT-PCR. Results:In PND 10 and PND 20, the expression levels of CaMKⅡ α and CaMKⅡ β mRNA in the hippocampus of offspring mice exposed to arsenite had no significant difference compared with those in control group. In PND 40, the expression levels of CaMKⅡα mRNA in the hippocampus of offspring mice exposed to arsenite were significantly lower than those in control group, the expression levels of CaMKⅡβ mRNA in the hippocampus of offspring mice exposed to 30 and 60 mg/L arsenite were significantly lower than those in control group (P<0.05) . Conclusion:Arsenite can affect the expression levels of CaMKⅡsubunits in the hippocampus of offspring mice and further may impair the learning and memory ability.